A New Purple Color Marker for Yeast
'' Dominant marker for rapidly screening plasmid maintenance using a visual or fluorescence assay in both Saccharomyces cerevisiae and Candida albicans.
Background: S. Cerevisiae and C.albicans yeast have been widely used as research tool that facilitates the scientific exploration of
many biological mechanisms, such as replication and metabolism. The unique characteristics of yeast organisms and plasmid borne color markers enable the examination of a variety of physiological properties providing an invaluable tool for researchers worldwide. However, molecular studies in C. albicans, a human fungal pathogen, have been hampered by the lack of plasmids that can autonomously replicate in this organism.
Technology Overview: Dr. Neta Dean from Stony Brook University has developed a yeast-codon optimized gene encoding the red fluorescent protein variant, mCherry (yEmRFP), that confers both a unique bright purple color and fluorescence to both C. albicans and S. cerevisiae. The yEmRFP plasmid-born biomarker allows for RFP expression in C. albicans, whose non-canonical genetic code has previously precluded expression of all forms of RFP. This yEmRFP can be used as a fluorescent protein tag for in vivo analysis of Candida proteins, or simply as a cell marker to monitor the fate of input pathogenic fungal cells within host tissue during an infection.
Advantages: Easy visual/fluorescence identification Applicable to any strain background without the need for chromosomal engineering
Applications: Research Tool: Genetic Screening, Yeast Biology Intellectual Property Summary: Issed US 8,921,045
Stage of Development: Plasmid available
Available for Licensing
Available for License