Extend the Field of View of Selective Plan Illumination Microscopy by Tiling the Excitation Light Sheet

Novel method for 3D imaging by SPIM with high 3D spacial resolution, good optical sectioning compatability, and a large field of view Background: Selective plane illumination microscopy (SPIM) is becoming increasingly attractive for 3D live fluorescence imaging in biological research. In comparison with conventional fluorescence imaging techniques based on the epi-illumination configurationSPIM gains its advantages by separating the sample excitation from the fluorescence detection using two objectives with optical axis orthogonal to each other, so that the excitation light is confined near the detectionfocal plane rather than passingthrough the entire specimen. By doing so, SPIM improves both the axial resolution and the optical sectioning capability, reduces the photobleaching and phototoxic effect, while still retaining its high imaging speed. Technology Overview: Dr. Liang Gao, Assistant Professor at Stony Brook University proposed a novel method for 3D imaging to achieve high 3D spacial resolution and good optical sectioning capability together with a large field of view.The proposed method can also be combined with other methods to further improve the imaging performance. Advantages: Improved imaging performance of current selective plane illumination microscopy techniques Can be combined with many of the current techniques to improve its performance Applications: Selective Plane Illumination Microscopy Intellectual Property Summary: PCT Patent Filed Stage of Development: Licensing Potential: We seek to develop and commercialize, by an exclusive or non-exclusive license agreement and/or sponsored research, with a company active in the area Licensing Status: Available for License Additional Information: Feild of view, Selective plan illumination, Microscopy, Light sheet https://stonybrook.technologypublisher.com/files/sites/medical-devices1a.jpg , https://stonybrook.technologypublisher.com/files/sites/optics-and-photonics-11.jpg
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