Geneneration of Permissive Cell Lines for Sequence-Attenuated Organisms

Novel method for allowing good growth of an attenuated organism Background: It is possible by deoptimization of codon bias, or codon pair bias, or by the insertion of toxic coding sequences to attenuate genes and organisms for use as live, attenuated vaccines. However, the degree of attenuation is limited by the viability of the organism; if the attenuation is too severe, the organism will not grow, or not grow sufficiently well, and so cannot be produced, studied, or manufactured. Thus, it is important to make cell lines tailored to be permissive to certain kinds of deoptimization, so as to allow good growth of highly attenuated organisms in a permissive cell line. Technology Overview: Dr. Bruce Futcher, professor in the Department of Molecular Genetics and Microbiology at Stony Brook University created a method for making a permissive cell line, allowing good growth of an attenuated organism. The permissive cell line is matched to the nature of the attenuating mutations. The permissive cell line is defective in quality control pathways, which would otherwise degrade the mutant, attenuated mRNA. Advantages: Allows highly attenuated organisms to be grown efficiently. Applications: Making a permissive cell line, allowing good growth of an attenuated organism. Intellectual Property Summary: Provisional Pending Stage of Development: In-vitro yeast data is available. Licensing Potential: We seek to develop and commercialize, by an exclusive or non-exclusive license agreement and/or sponsored research, with a company active in the area. Licensing Status: Available for License Additional Information: Gene, Sequence, Encoding, De-optimization, Attenuation, Vaccine
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