Molecular Roadblocks for RpoN Binding Sites

Background:

The RNA polymerase sigma-54 factor (rpoN) gene is conserved across a wide number of bacterial species, and the RpoN protein has been shown to activate transcription of genes encoding proteins involved in nitrogen utilization, pilin, flagella, and virulence-related factors in bacteria.

Technology Overview:

Novel synthetic peptides have been developed by SUNY ESF researchers to downregulate the activity of the RpoN protein. The peptides repress virulence and increase antimicrobial susceptibility in bacteria, demonstrating infection control activity.

Advantages: 

• Broad-spectrum: The RNA polymerase sigma-54 factor (rpoN) gene is widely conserved among bacterial species.
• Versatile: Could be used in combination with a wide range of antimicrobials and disinfectants.

Applications: 

• Human health (oral health, chronic infections, wound care, Cystic Fibrosis, surgical site infections, implanted medical devices).
• Animal health (oral health, skin health, and wound care).
• Disinfection (medical and industrial).
• Anti-fouling and anti-corrosion (food processing, water purification and distribution, cooling towers, oil and gas pipelines, paper and pulp installations).