Detection of Biomolecule Carbonylation

 Background: 

Oxidative stress is a characteristic of many disease states. One result of oxidative stress is an irreversible reaction of radicals with biomolecules that results in covalent addition of carbonyls to the biomolecules. The presence, location and amount of carbonylation is correlated with cellular and tissue damage, which can be used in for diagnostics, drug discovery, early state toxicity screening, and [possibly] in vivo imaging.

 Technology Overview: 

We have developed a novel fluorescent biocompatible probe to detect oxidative damage in live cells and tissues in as little as 5 minutes. The probe outperforms various commercially available toxicity and reactive oxygen species assays, demonstrating superior sensitivity and signal-to-noise ratios.   

 Advantages: 

• Sensitive
• Rapid
• Live cell and tissue compatible
• Cellular damage can be detected at lower toxin concentrations
• Detection of an early biomarker for disease, toxicity, cell and tissue damage
• May be used on existing biopsy material