A New Bioorthogonal Reaction
The versatile biomolecule coupling technology is based on a bioorthogonal reaction using formylphenylboronic acid click reagents. A boronic acid or ester having an ortho carbonyl or aldehyde on an optionally substituted aryl or aromatic heterocyclic compound reacts with an alpha-effect amine under biologically compatible conditions. The resulting dehydration reaction, forming a hydrazono or imino arylboronic acid product, is frequently followed by a second reaction to form a heterocyclic ring that contains a boron atom.
- Rapid: Reaction occurs in minutes at neutral pH and room temperature.
- Stable: High solubility and serum stability of therapeutic antibody conjugates.
- Tunable: Control of conjugate stability via informed selection of features of reactive pair.
Binghamton University RB395